Kai Cheng Yang
National Chiayi University Taiwan.
Title: In vitro and in vivo study to evaluate the inhibitory effect of anthocyanins extract from black soybean seedcoat toward uric acid level and uricase activity
Biography
Biography: Kai Cheng Yang
Abstract
This study aimed to evaluate the effects of anthocyanins extract from black soybean seedcoat on xanthine oxidase’s activity and the uric acid contents in zebrafish (AB strain). In the vitro experiment, the inhibitory effect of the extract toward xanthine oxidase was determined as well as the kinetic of the inhibition was evaluated. For in vivo experiment 5 days post fertilization zebrafish was normally develop and randomly divided into blank group, hyperuricemia group (200 μmol/L (potassium oxonate (PO)+10 μmol/L (xanthine sodium salt (XSS)), anthocyanin group (200 μmol/L PO+10 μmol/L XSS+different concentrations of the extract ) and positive control group (200 μmol/L PO+10 μmol/L XSS+allopurinol 136 mg/L). The uric acid level and uricase activity were measure after cultivation for 28.5 ℃ 24hr. Our data indicated that the black soybean extract showed a mixed type inhibition toward xanthine oxidase activity. In vivo experiment, all anthocyanin groups effectively reduce uric acid level and inhibit uricase activity in hyperuricemia zebrafish. Therefore, anthocyanins extracts form black soybean seedcoat show a potential for high uric acid prevention