Eliana Yissel Aguilera Angel
Pontificia Universidad Javeriana, Colombia
Title: Chromatographic analysis of intact glucosinolate in leaves and flowers from Colombian nasturtium (Tropaeolum majus)
Biography
Biography: Eliana Yissel Aguilera Angel
Abstract
Nasturtium (Tropaeolum majus L.) is an edible plant that grows wildly in South America, it is a plant with a high functional potential due to its content of bioactive compounds such as flavonoids, carotenoids and, glucosinolates. Nasturtium bioactive compounds have been related with anti-inflammatory activity, modulation of glucose metabolism and antioxidant activity. Nowadays the use of nasturtium is mainly ornamental for that reason it is important to promote its study to know its content of bioactive compounds and its functional potential. Therefore, the aim of this study was to evaluate qualitative and quantitative the intact glucosinolates (Gls) content in freeze-dried leaves and flowers nasturtium collected in Cogua - Cundinamarca, Colombia . Intact GLs were evaluated by HPLC-DAD method using the extract methodology proposed by Förster et al (2015) without sulfatase treatment to obtain desulfoglucosinolates (which can cause degradation of the structure of some glucosinolates, forming artifacts) . The content of intact glucosinolates was 3,03±0,49 mg/g dry weight (DW) from leaves and 4,58±0,37 mg/g DW from flowers. The result agrees with that reported by Schreiner, et al., 2009 , where 3.13 mg/g DW from leaves nasturtium was found by desulfoglucosinolates method. Then is possible determine the content of glucosinolates in nasturtium using a faster methodology, obtaining results comparable to those reported by the standardized method of quantification of desulfoglucosinolates. This study contributes with the knowledge regarding the methodologies for the determination of intact glucosinolates in different plant matrices and to the study of the Colombian nasturtium as source of bioactive compounds. In conclusion the determination of intact glucosinolates content is a quick method which facilitates the: i) nasturtium study, ii) quantification of glucosinolates rich extracts and products, and iv) promotes its use for supplements, phytoterapeutics , foods, and cosmetics development.